Skip to main content
Fig. 6 | BMC Molecular and Cell Biology

Fig. 6

From: Increased expression of MUSASHI1 in epithelial breast cancer cells is due to down regulation of miR-125b

Fig. 6

MSI1 mRNA and protein expression analyzed in breast cancer cell lines. MSI1 expression analyzed in (a) MCF-7 and (b) T-47D breast cancer cells transfected with miRNA precursor plasmids and compared to cells transfected with a scrambled negative control miRNA. c MSI1 protein expression analyzed in MCF-7 and (d) T-47D breast cancer cells transfected with miRNA precursor plasmids as compared to cells transfected with a scrambled negative control miRNA (Control miRNA). Error bars denote S.E.M. for three independent repeats in each experiment. The expression of mRNA was analyzed using RT-qPCR, and normalized to GAPDH in comparison to untransfected cells. MSI1 protein expression was analyzed using western blotting Error bars signify S.E.M. for three independent repeats in each experiment. One-way ANOVA indicated differences (*p < 0.05)

Back to article page

BMC Molecular and Cell Biology

ISSN: 2661-8850

Contact us