Fig. 6

Expression of WNT and EGF pathways markers in cIECs cultured with (GF) and without (CTR) growth factors. AXIN2, LEF1 = Lymphoid Enhancer Binding Factor 1; c-Myc = MYC proto-oncogene bHLH transcription factor; MAPK1 = Mitogen-activated protein kinase 1; EGFR = Epidermal Growth Factor Receptor. Data are reported as 2^−DDCt values calculated by Delta–Delta Ct (DDCt) method. Expression was normalized to RPLP0, and data are represented as means ± SEM (n = 3) relative to the CTR group (represented with a dashed line). Significant differences between CTR and GF group (t-test) are represented as * (p < 0.05)