Table 1 Recipes of different media used during isolation protocol

Digestion medium

• Dulbecco modified eagle’s medium high glucose (Cat.# D1145 - Sigma-Aldrich St. Louis, Missouri, USA);

• 1% fetal bovine serum (Cat. # F7524 - Sigma-Aldrich, St. Louis, Missouri, USA).

• 200 U/mL collagenase (Cat. # C9407 - Sigma-Aldrich, St. Louis, Missouri, USA).

• 100 U/mL hyaluronidase (Cat. # H3506 - Sigma-Aldrich, St. Louis, Missouri, USA).

• 1x penicillin-streptomycin (Cat. # P4333 - Sigma-Aldrich, St. Louis, Missouri, USA).

Washing medium

• Dulbecco modified eagle’s medium high glucose.

• 1% fetal bovine serum;

• 2% w/v sorbitol (Cat. # S1876 - Sigma-Aldrich, St. Louis, Missouri, USA).

• 1x penicillin-streptomycin.

Basal medium

• Dulbecco modified eagle’s medium high glucose (DMEM).

• 2% fetal bovine serum;

• 1x penicillin-streptomycin;

• 10 mM L-glutamine (Cat. # G7513 - Sigma-Aldrich, St. Louis, Missouri, USA).

• 10 μg/mL insulin (Cat. # I6634 - Sigma-Aldrich, St. Louis, Missouri, USA).

• 25 ng/mL human epidermal growth factor (Cat. # SRP3196 - Sigma-Aldrich, St. Louis, Missouri, USA)

• 1 mM sodium pyruvate (Cat. # P5280 - Sigma-Aldrich, St. Louis, Missouri, USA).

• 0.1 mg/mL heparin sodium salt from intestinal porcine mucosa (Cat. # H3149 - Sigma-Aldrich, St. Louis, Missouri, USA).

• 1x non-essential amino acids (VWR, www.wvr.com– Cat.# X0557);

• 1 mM sodium butyrate (Cat. # B103500 - Sigma-Aldrich, St. Louis, Missouri, USA).

• 0,081 mg/L putrescine (Cat. # P5780 - Sigma-Aldrich, St. Louis, Missouri, USA).

• 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES - Cat. # H4034 - Sigma-Aldrich, St. Louis, Missouri, USA).