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Table 2 Recipes of different media based on time post-seeding

From: Isolation, culture, and characterization of chicken intestinal epithelial cells

 

BUTYRATE

R-SPONDIN 1

NOGGIN

hEGF

Y-27632

CHIR99021

First 48 h

YES (1 mM)

NO

NO

YES (25 ng/mL)

YES (10 μM)

NO

After 48 h

NO

YES (500 ng/mL)

YES (100 ng/mL)

YES (50 ng/mL)

NO

YES (3 μM)

  1. After the first 48 h, the medium change was performed every other day. These supplements were added to the basal medium. R-spondin1 (Cat.# SRP3292 - Sigma-Aldrich, St. Louis, Missouri, USA), Noggin (Cat.# 21–7075-U020 - Tonbobio, San Diego, California, USA), CHIR99021 (Cat.# SML1046 - Sigma-Aldrich, St. Louis, Missouri, USA), Y-27632 (Cat.# Y0503 - Sigma-Aldrich, St. Louis, Missouri, USA)
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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