Table 3 Primer list used to characterize cIECs designed with PrimerBLAST
From: Isolation, culture, and characterization of chicken intestinal epithelial cells
Gene | Primer sequence (5′- > 3′) | Product length (bp) | Accession N. | |
|---|---|---|---|---|
Epithelial markers | E-cadherin | F: TGAAGACAGCCAAGGGCCTG R: CTGGCGGTGGAGAGTGTGAT | 109 | NM_001039258 |
Villin | F: GAACCTCTCGTGGCACCGC R: CTCATGTCCCTGCACCTCCC | 152 | XM_418521.5 | |
Cytokeratin 18 | F: CACAGATCCGGGAGAGCCTG R: CTCCACCGCGCTGTCATAGA | 110 | XM_025145666 | |
Cytokeratin 20 | F: GCGCGTTATAAAGGAGGAGCTG R: CGCTGATTTACGGGCCGAAC | 200 | NM_204749.2 | |
Zonula occludens-1 | F: TCTGCACAGTGAGGTTGGCT R: GGCTGTCCTGCATCGGTGT | 145 | XM_004934975 | |
Occludin-1 | F: TGCTTTTGCCCAAGCAGGAA R: TGTGGGAGAGGCACCAGTTG | 153 | NM_204417 | |
Claudin-1 | F: TCGGTGGTGGTCACTTCGTC R: CGCTGATTTACGGGCCGAAC | 113 | NM_001004768 | |
Staminal markers | LGR5 | F: TGGGCTCCACAGCCTAGAGA R: CCTACAAACGCACGCTCAGG | 144 | XM_425441.5 |
SOX9 | F: CCACCATGTCGGATGACTCC R: GGTGTTCTCTTGAGGTCGGG | 86 | NM_204281.1 | |
CDXA | F: CAGTGAGTGTCCCCCATGTC R: GGGACAGATGTCTGCAGGTC | 92 | NM_204676.2 | |
CDXB | F: ATCTGGTTCCAGAATCGCCG R: TGGTGGGAACAGGGAACTTG | 141 | NM_204614.1 | |
Endothelial cell markers | CD34 | F: TCTGCACAGTGAGGTTGGCT R: GGCTGTCCTGCATCGGTGT | 153 | XM_004934975 |
CD45 | F: TCCGACGCAGAGTGAATGCT R: AGCCCCTCCAACATAGCGTC | 118 | NM_204417.2 | |
CD146 | F: TCACAAAGCCAGAGGCACGTA R: AGCAGGAAAGGGTCGAAGGA | 196 | NM_001004768 | |
CD90 | F: GAACGTCTACCGGAACCGAG R: GTGTAGTCGTTGGTGGCCTT | 126 | NM_204381.2 | |
CD73 | F: GGGCACTCTGGCTTTACTGT R: GGAGGGAGGGGTTCCTGTAT | 111 | XM_004940396 | |
CD105 | F: GCGAAAATAGCAACAGCCCC R: GTACAGCCCTTCACCTCACG | 103 | NM_001080887 | |
WNT and EGF pathways | AXIN2 | F: ACCGAGACGTTGGAGAACGG R: ATCCGTCAGGGCATCACTGG | 138 | NM_204491.1 |
c-MYC | F: CTGGTCCTCAAGCGGTGTCA R: GACCCTGCCACTGTCCAACT | 114 | XM_015283089 | |
LEF1 | F: CCCACCCATCTCACATCCCC R: GTGAAAACCAGCCAAGAGGTGG | 141 | NM_205013.2 | |
MAPK-1 | F: TGTGACTTCGGACTGGCTCG R: AGGAGCCCTGTACCAACGTG | 93 | XM_015275131 | |
Cyclin-D1 | F: CGAGGTGGAGACCATCCGAC R: GAAGTAGGACACCGAGGGCG | 112 | NM_205381 | |
Cyclin-E | F: CGTTCCTCCAGGATCCCGAC R: CCAGGACAGCTGGTTTTCGT | 80 | NM_001031358 | |
EGFR | F: CCGCAGCTGGGTAGGCAC R: GCTCCTCACTCTGCACTGCT | 129 | NM_205497 | |
Reference | RPLP0 | F: TCACGGTAAAGAGGGGAGGTG R: CTTGCTCAGTCCCCAGCCTT | 143 | NM_205179 |