Table 4 Antibodies used for immunofluorescence characterization
From: Isolation, culture, and characterization of chicken intestinal epithelial cells
Reagent | Dilution | Product catalog number |
|---|---|---|
Mouse anti-chicken cytokeratin 18 | 10 μg/mL | MA1–06326 |
Rabbit anti-chicken zonula occludens 1 | 10 μg/mL | 61–7300 |
Rabbit anti-chicken pan-occludin | 2 μg/mL | 71–1500 |
Mouse anti-chicken vimentin | 2 μg/mL | MA5–11883 |
Rabbit anti-villin | 10 μg/mL | ab130751 |
Rabbit anti-MAPK1/ERK2 | 1:100 | MA5–15134 |
Mouse ant-c-Myc | 10 μg/mL | MA1–16637 |
Donkey anti-mouse secondary antibody, TRITC conjugated | 4 μg/mL | A16016 |
Goat anti-rabbit secondary antibody, FITC conjugated | 4 μg/mL | A27034 |