Fig. 1

STAU2 depletion facilitates cell growth of hTERT-RPE1 cells. Growth curves (left panels) and colony growth assays (right panels) were used to monitor cell proliferation. The relative growth of wild-type cells was arbitrary fixed to 1 for the colony assays. *** p-value ≤0.001; ** p-value ≤0.01; * p-value ≤0.05. Two-tailed Student’s t-test for growth curves analysis. One sample t-test for the colony assay. (Middle) Representative Western blots showing STAU2 expression. a STAU2-KO clones (A4, B5, C6) and wild-type (WT) hTERT-RPE1 cells. n = 4. b hTERT-RPE1 cells were infected with viruses expressing shRNA control (shNT) or shRNA against STAU2 (shSTAU2). n = 5. c HeLa cells were infected with viruses expressing shRNA control (shNT) or shRNA against STAU2 (shSTAU2). n = 5. d CRISPR-mediated STAU2-KO HCT116 (STAU2-KO) and CRISPR-treated cells that still express STAU2 as controls (Ctrl). n = 5