Fig. 3

Identification of proteins in proximity to STAU2. a Control (empty vector), STAU2⁵²-myc₃ and STAU2⁵²-BioID2-HA infected hTERT-RPE1 cells were analyzed by immunofluorescence using anti-Myc and anti-HA antibodies to compare the subcellular localization of STAU2-tagged proteins. Both fusion proteins are cytoplasmic and mostly excluded from the nucleus, as expected. DAPI was used to stain the nucleus. b hTERT-RPE1 cells were infected with viruses expressing an empty vector or STAU2⁵²-BioID2-HA. Two days post selection, cells were incubated in the absence (−) or presence (+) of 50 μM biotin for 16 h. Biotinylated proteins were visualized by Western blotting using streptavidin-coupled HRP. c 60 proteins with the best interaction probability scores (decreasing fold-change from top to bottom) are listed along with a heat map (right) representing the average spectral counts of STAU2 interactors. d Pie chart of the percentage of STAU2 interactors associated with different biological processes. Percentages represent the number of specific interactors in each molecular process/total number of specific interactors