Fig. 2
From: A cell-based ribozyme reporter system employing a chromosomally-integrated 5′ exonuclease gene
Phenotypic analysis of plasmid transformants. a Western immunoblot analysis to detect RNaseJ1-6xHis in E. coli DH5α harboring pBAD33 (pBAD33, lane 1–3) and pBAD33-rnjA-6xHis (pRJ1, lane 4–6) cultured in 0, 0.0012, and 0.0037% (w/v) arabinose as shown by the wedge above the lanes. Total protein-stained membrane is shown in the left panel and immunodetection of RNaseJ1-6xHis using Anti-6X His IgG, CF™680 (Sigma-Aldrich, Merck KGaA, Germany) is shown in the right panel. The expected size of RNaseJ1-6xHis is 63 kDa, as shown by the arrow. Lane M indicates KaledioscopeTM prestained protein ladder (Bio-Rad). b Growth analysis of E. coli BL21(DE3) co-transformants of pRSETC/ pBAD33-rnjA-6xHis (pRSETC_pRJ1), pEGFP-hDHFR/ pBAD33-rnjA-6xHis (pEGFP-hDHFR_pRJ1), pRzIEGFP-hDHFR/ pBAD33-rnjA-6xHis (pRzI_pRJ1), pRzIIEGFP-hDHFR/ pBAD33-rnjA-6xHis (pRzII_pRJ1). OD600 was measured every hour from 0 to 8 h cultivation time in cultures grown in HDAG medium with no arabinose and data were plotted using the Growthcurve package in R software. Points represent mean of 2–4 experiments and error bars represent 95% confidence intervals