Fig. 5
From: A cell-based ribozyme reporter system employing a chromosomally-integrated 5′ exonuclease gene
Growth analysis and fluorescence intensity of iRJ1 double integrants. a Growth analysis of double integrants ΔarsB::EGFP-hDHFRΔlacZ::RJ1 (iEGFP-hDHFR_iRJ1), ΔarsB::RzIEGFP-hDHFRΔlacZ::RJ1 (iRzI_iRJ1), ΔarsB::RzIIEGFP-hDHFRΔlacZ::RJ1 (iRzII_iRJ1), ΔarsB::glmSEGFP-hDHFRΔlacZ::RJ1 (iglmS_iRJ1), and ΔarsB::M9EGFP-hDHFRΔlacZ::RJ1 (iM9_iRJ1). OD600 was measured every hour from 0 to 8 h cultivation time and data were plotted using the Growthcurve package in R software. Data are shown for each cell type grown in the presence or absence of 0.0012% (w/v) arabinose. Points represent the mean of 4–12 experiments and error bars represent 95% confidence intervals. P-values from two sample t-tests for iEGFP-hDHFR_iRJ1, iRzI_iRJ1, iRzII_iRJ1, iglmS_iRJ1, and iM9_iRJ1 integrants comparing growth in the presence or absence of 0.0012% (w/v) arabinose are 0.07435, 0.3102, 0.1096, 0.3532, and 0.345, respectively. b Fluorescence intensity of double integrants cultured in 0.0012% (w/v) arabinose relative to untreated control. Box plots show relative fluorescence data distribution. Dots indicate relative fluorescence data from individual experiments, and median values are indicated by the bold black line. P-values from single value two-tailed t-tests comparing group mean to 1 of iEGFP-hDHFR_iRJ1, iRzI_iRJ1, iRzII_iRJ1, iglmS_iRJ1, and iM9_iRJ1 are 0.5759, 0.0002, 0.0261, 0.0055, and 0.4059, respectively