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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Association of Sonic Hedgehog with the extracellular matrix requires its zinc-coordination center

Fig. 2

ECM-association of ShhN requires zinc and the catalytic E177V residue. a Diagram of the Shh structure (PDB: 3D1M) with relevant residues indicated. b Western blot analysis of the lysate and ECM of Hek293t cells transfected with Shh-C199* and Shh-C199*/E177V and cultured in DMEM containing 0.18 mM calcium and the indicated concentrations of zinc. Uncropped blots can be found in the supplement. c Quantification of band intensities shown in C as a fraction of ECM over lysate, normalized to the respective mock treatment to better illustrate the effects of zinc treatment. n = 2. d Hh response in LightII cells grown on the conditioned and decellularized ECM of Hek293t cells transfected with Shh-C199* or Shh-C199*/E177V. n ≥ 3, *p < 0.05, ****p < 0.0001. Full-length blots are presented in Supplementary Figure 4

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