Fig. 4
From: Association of Sonic Hedgehog with the extracellular matrix requires its zinc-coordination center
The zinc-coordination domain of BacHh is not sufficient for association with the ECM. a Western Blot analysis of the lysate, ECM, and supernatant of ShhN-HA or Shh-BacHh-HA (diagrams) transfected Hek293t cultured in the indicated zinc concentrations. Uncropped blots can be found in the supplement. b Detergent-free live staining with an α-HA antibody (3F10) of transfected Ptch1LacZ/LacZ;Ptch2−/−;Boc−/−;Cdo−/−;Gas1−/− cells. Nuclei were stained with DAPI. Uncropped, full-length blots are presented in Supplementary Figure 6