Fig. 5
From: Association of Sonic Hedgehog with the extracellular matrix requires its zinc-coordination center
Calcium alters the sensitivity of Shh to zinc. a Western blot analysis of lysates and ECM of Hek293t cells transfected with Shh-C199* and Shh-C199*/E90A/E91D/D96AD130N/D132L, or Shh-C199* and Shh-C199*/E90A/E91D/D96A/E127A/D130N/D132L (CaFree), cultured in the presence of 0.18 or 1.8 mM calcium, and in the absence or presence of 5 μM added zinc. b ECM-associated Shh-C199*N or Shh-C199*/CaFree deposited by transfected Hek293t cells was assessed by ELISA in the presence of .18 mM calcium (left panel) or 1.8 mM calcium (right panel) and increasing zinc concentrations as indicated. Shown are means and standard errors, n = 6. Uncropped, full-length blots are presented in Supplementary Figure 7