Fig. 2

Endogenous Cyr61 is required for the proliferation and migration of SCs and regulated c-Jun expression. a Histograms for three independent qPCR experiments of primary SCs transfected with Cyr61-specific siRNAs or with NTC siRNA; b Western blots demonstrating protein knockdown efficiency of three different Cyr61-targeting siRNAs; c Histograms for three independent western blot experiments of primary SCs were transfected with Cyr61-specific siRNAs or with NTC siRNA; d ELISA histograms of the results from three independent experiments for Cyr61 secreted by SCs after transfection with Cyr61-specific siRNA or with NTC siRNA; e Histograms of three independent experiments of SCs cultured for 60 h after transfection with Cyr61-specific siRNAs or with NTC siRNA; f Representative images of SCs transfected with siRNAs that migrated to the underside of transwell membranes. g Histogram results from three independent experiments for cell migration. h Western blots demonstrating c-Jun protein expression in SCs and SCs transfected NTC siRNA or Cyr61-specific siRNAs. i Histograms for three independent western blot experiments of c-Jun expression in SCs and SCs transfected with Cyr61-specific siRNAs or with NTC siRNA. *, p < 0.05, **, p < 0.01, ***, p < 0.001, ****, p < 0.0001