Fig. 3

Exogenous Cyr61 increases the proliferation and migration of SCs and upregulated c-Jun expression. a Histogram of SC proliferation cultured for 48 h after the addition of 0.25 nM, 0.5 nM, 1 nM, 2 nM of Cyr61 and control media measured using CCK-8 assays; b Histogram of SC proliferation cultured for 36 h after the addition of 2 nM of Cyr61, 0.5 μg neutralizing antibody αvβ3 and Mouse IgG1 Isotype Control (IgG), 2 nM Cyr61 + 0.5μg neutralizing antibody αvβ3 and control media measured using CCK-8 assays; c Histogram of SC proliferation cultured for 60 h after the addition of 2 nM of Cyr61, 0.5 μg neutralizing antibody αvβ3 and Mouse IgG1 Isotype Control (IgG), 2 nM Cyr61 + 0.5 μg neutralizing antibody αvβ3 and control media measured using CCK-8 assays; d Representative images of SCs treated with 2 nM of Cyr61, 0.5 μg neutralizing antibody αvβ3 and Mouse IgG1 Isotype Control (IgG), 2 nM Cyr61 + 0.5 μg neutralizing antibody αvβ3 and control media that migrated to the underside of the transwell membrane after 24 h. e Histograms from three independent cell migration experiments; f Western blots demonstrating c-Jun protein expression in SCs treated with 2 nM of Cyr61 or control medium; i Histograms for three independent western blot experiments of c-Jun expression in SCs treated with 2 nM of Cyr61 or control medium. *, p < 0.05, **, p < 0.01, ***, p < 0.001, ****, p < 0.0001