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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: The Glypican proteoglycans show intrinsic interactions with Wnt-3a in human prostate cancer cells that are not always associated with cascade activation

Fig. 5

Immunoblot assays of RWPE-1, PC-3, DU-145, and LNCaP cells, evaluating Wnt-3a, and β-catenin. LNCaP cells were not analyzed using the Wnt-3a antibody. a Immunoblot results for Wnt-3a, cellular, cytoplasmic and nuclear β-catenin for RWPE-1, PC-3, DU-145, and LNCaP. Housekeeping proteins such as GAPDH and Lamin A were also investigated. b Densitometric analyses of the immunoblots, where data are normalized against GAPDH, except for nuclear β-catenin, which was normalized against Lamin A. Data are plotted as the mean ± s.e.m., and two independent experiments were performed. Prostate cancer cells were compared to RWPE-1 cells using Dunnett’s test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

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