Fig. 1

Preparation of the leukocyte-depleted PRP-based skin equivalent. The platelet rich plasma (PRP) was mixed with 20 mM calcium chloride (CaCl2) solution and 6 × 104 cells/cm2 fibroblasts. The mixed solution was poured into a 24 mm trans-well at 37 °C in a CO2 incubator for 24 h. The CaCl2 worked as activator for the formation of autologous thrombin and allowed the PRP-based derma equivalent to solidify. At day 2 the epithelial cells were seeded on the PRP-based derma equivalent with a density of 6 × 104 cells/cm2. This skin model was cultured with an air-liquid interface system allowing the development of a multi-layered skin equivalent