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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Novel leukocyte-depleted platelet-rich plasma-based skin equivalent as an in vitro model of chronic wounds: a preliminary study

Fig. 4

IL8 and TGFA Gene Expression. Bar graphs showing the significant differences between the three conditions studied: control, scratched and treated, in relation to log fold change expression levels of the genes IL8 (a) and TGFA (b). Bar graphs showing log fold change expression levels of the genes IL8 (c) and TGFA (d) of the control, scratched and treated conditions for all time points at which they were measured. The mRNA expression was determined by qPCR. Relative transcript levels were normalized to the ACTB reference gene. The expression levels of the skin models at baseline time point, before scratching, were used as a calibrator. Data presented showing the log2 fold change. (2−ΔΔCt) method. The data are presented as mean ± standard deviation (SD). P values were worked out through a paired student t-test. p < 0.05 was considered as statistically significant and is shown in the figure where applicable (* p < 0.05, ** p < 0.01, *** p < 0.001)

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