Table 2 Primer sequences and amplicon sizes for each gene analysed in the study
Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | Fragment size |
|---|---|---|---|
CD34 | TGAAGCCTAGCCTGTCAC | ATAAGACCTCCAGCTGTGCG | 180 bp |
CD45 | GTGTTTCATCAGTACAGACG | GCTGTCATTTCAACCACAAC | 191 bp |
CD73 | ATGGTGTGGAAGGACTGATC | CATCGCTCAGAAAGTGAGG | 310 bp |
CD90 | TGCTCTTTGGCACTGTGG | CTGCTCCTGCTCTCCCTCT | 248 bp |
CD105 | GGGGTCAACACCACAGAG | CACATCCTGAGGGTCCTG | 261 bp |
IL8 | GAGAGTGATTGAGAGTGGACCAC | AAACTGGGTGCAGAGGGTTGTG | 90 bp |
TGFA | GGTCCGAAAACACTGTGAGTGG | AAGAGCCCAGAGGAGGAGTTTG | 108 bp |
ACTB | AGTCCTAGCTACTCCGGAGGC | CGGCTATTCTCGCAGCTCAC | 113 bp |