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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: Assessing nucleic acid binding activity of four dinoflagellate cold shock domain proteins from Symbiodinium kawagutii and Lingulodinium polyedra

Fig. 5

Competition assays of SkCSP1 with ssDNA, dsDNA and RNA. Symbiodinium CSP1 binds to ssDNA better than dsDNA. Cold oligos have a different sequence than the ssDNA. Concentration of SkCSP1 is 0.5 μg in all the assays; position of the shifts are shown by arrows. RNA competes efficiently with both dsDNA and ssDNA. The black triangle shows the different concentrations of the unlabeled nucleic acids (1x, 10x, 30x, and 80x the probe concentration)

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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