Fig. 1

Construction of the SDHD mutant. The gene sequence of SDHD with CRISPR targeting sites is shown a. The sgRNA and PAM sequences predicted by the online idtdna program are depicted in blue and red colors, respectively; the specific site for mutation (at 118-bp) is shown with a green arrow. Expression of SDHD protein is shown b. The protein extracts reacted with rabbit polyclonal antibodies to SDHD and rabbit polyclonal antibodies to β-Actin are shown. The DNA level recombination events in the genome of the mutant are illustrated c. The bases c and a (highlighted and in purplr color) of the parent have been replaced with the base g (highlighted and in red color) in the mutant. Expression of representative subunits from all five ETC complexes are shown d-i. The expression of NDUFB8 (complex i; d and e), SDHB (comlex II; d and f), UQCRC2 (complex III; d and g), MTCO1 (complex IV; d and h) and ATP5A (complex V; d and i) were normalized to the expression of β-Actin