Fig. 2

ROS production, apoptosis, and necrosis of cell lines. ROS generation measured using ROS-Glo H₂O₂ Assay of parent HEK293 and the mutant HEK293ΔSDHD is shown (A). The measurements are expressed as total luminescence per 15,000 cells measured at 0, 24, and 72 hours after addition of substrate to the reaction. Apoptosis (B) and necrosis (C) per 15,000 cells measured using RealTime-Glo Annexin V Apoptosis and Necrosis Assay at 0, 2, 24 and 72 hours of incubation are shown. The apoptosis measurements are expressed as total luminescence, whereas necrosis measurements expressed as total flourescence. Error bars represent the SE of the mean