Fig. 6

2D-PLS-DA score plot of rat serum metabolites treated with VOA and TMX. The recorded serum metabolomics data were first analyzed with combined 2D PLS-DA to see the variation in the principal components. The 2D PLS score plots of toxic control (Group 2) and that Group 4 animals treated with MNU dose depicting a clear separation from the normal, VOA low dose (G3), TMX treated (Group 5), and with a combination of both of these drugs (Groups 6). A metabolic profile of Group 4 animals was observed like that of toxic control animals after treatment with a high dose of VOA, which indicates its toxicity at this dose. This means VOA low dose and its combination with somehow resetting back the perturbed metabolites due to MNU administration excluded from the data matrix to evaluate the discriminatory significance of aromatic residues