Fig. 4
From: Chick fetal organ spheroids as a model to study development and disease
RT-PCR of day 8 FOSs revealed that mRNA expression of typical in vivo organ characteristic genes are enriched in the corresponding FOSs. a GFAP expression in brain, but not in heart or liver FOSs/organs. b SP-C expression in lung, but not in heart or stomach FOSs/organs. c TNNT2 expression in heart, but not in brain or stomach FOSs/organs. d Alb expression in liver, but not in lung or heart FOSs/organs. e Barx1 expression in stomach, but not in brain or lung FOSs/organs. f SI expression in intestine, low in brain and none in heart FOSs/organs. g EDMTFH expression in epidermis, and minimal in lung or liver FOSs/organs. h, i The house-keeping gene GAPDH was used as a reference gene. White arrowheads highlight the lower, but consistent observed expression of GFAP and EDMTFH in brain and epidermal FOSs, respectively. a-i The first and last lane of each gel image show partial views of the ladder. The presented PCR-gel images are cropped, with full-length gels shown in Fig. S5