Fig. 6
From: Chick fetal organ spheroids as a model to study development and disease
Typical cell marker expression detected by immunocytochemistry in day 8 fetal brain, lung and stomach spheroids. a, b Expression of GFAP, indicating astroglial cell types, and HuC/D, marking post-mitotic neurons, detected in FOSs derived from the brain (n = 9). c, d Expression of SP-C, defining Clara and/or alveolar type II cells, and Nkx2.1, indicating lung bud formation, observed in fetal lung spheroids (n = 6). e Expression of Somatostatin, defining δ-cells, detected in fetal stomach spheroids (n = 6). The right panels indicate a magnified view of the area highlighted by white dotted boxes in the middle panels. a-d DAPI indicates nuclei. Scale bars: 100 μm