Fig. 3

Myoblasts derived from satellite cells of M. rhomboideus of 5-day-old piglets were seeded on gelatin-coated dishes and permanently cultivated at 35 °C or at 41 °C for 24 h or 48 h. Images of living cells (A-D) were taken with a Primovert microscope and Axiocam ERc5s (Carl Zeiss AG, Oberkochen, Germany). A staining for actin filaments with Phalloidin CruzFluor™ 594 Conjugate (red) and 4′,6-Diamidin-2-phenylindol (DAPI) for the nuclei (blue) was performed. Images of the phalloidin and DAPI stainings were taken with Leica DM 2400 fluorescence microscope (Leica Microsystems, Wetzlar, Germany). A higher magnification was presented in the inserts