Effects of temperature on proliferation of myoblasts from donor piglets with different thermoregulatory maturities

Table 1 Average cell index parameters (least square means ± standard errors) generated from real-time monitoring of 72 h proliferative growth

Parameter	Temperature (T)				Pool (P)		T P	P P	T × P P
Parameter	35 °C	37 °C	39 °C	41 °C	5	20	T P	P P	T × P P
Cell index (arbitrary units)	1.67 ± 0.12	1.63 ± 0.12	1.58 ± 0.12	1.37 ± 0.12	1.47 ± 0.09	1.65 ± 0.09	0.905	0.696	0.978
Slope (1/h)	0.048 ± 0.004	0.045 ± 0.004	0.042 ± 0.004	0.039 ± 0.004	0.041 ± 0.002	0.045 ± 0.002	0.323	0.297	0.385
Doubling time^a (h)	20.4 ± 1.2	22.7 ± 1.3	20.6 ± 1.3	21.8 ± 1.3	23.3 ± 0.9	19.4 ± 0.9	0.524	0.007	0.934

^a The doubling time was calculated over a 67 h period (from 5 to 72 h), starting at 5 h to allow the myoblasts to attach after seeding

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