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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Construction and characterization of EGFP reporter plasmid harboring putative human RAX promoter for in vitro monitoring of retinal progenitor cells identity

Fig. 1

Overview of retinal differentiation and characterization of hPSCs-derived RPCs. A Stepwise process of hPSCs differentiation into RPCs. B Phase contrast images of hPSCs cultured on Matrigel coated dish, C hPSC derived EBs cultured in low adherent dishes, D Expansion of RPCs from EBs on Matrigel coated dishes and (E) Expansion of hRPCs 21 days after seeding. F Immunocytochemistry of eye field-associated transcription factors: RAX, PAX6, LHX2 (red) and NESTIN (green). Scale bars: 100 μm and 50 μm (G) qPCR analysis of stemness and EFTF biomarkers in hPSCs and RPCs (*p < 0.05 vs control, n = 3)

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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