Construction and characterization of EGFP reporter plasmid harboring putative human RAX promoter for in vitro monitoring of retinal progenitor cells identity

Table 1 List of primers for amplification and characterization

Putative Promoter regions /Genes	Primers	Sequences (5′ > 3′)
OCT4	Sense Anti-sense	TCTATTTGGGAAGGTATTCAGC ATTGTTGTCAGCTTCCTCCA
NANOG	Sense Anti-sense	CAGCTACAAACAGGTGAAGAC TGGTGGTAGGAAGAGTAAAGG
NESTIN	Sense Anti-sense	TCCAGGAACGGAAAATCAAG TTCTCTTGTCCCGCAGACTT
SIX3	Sense Anti-sense	TCCTCCTCTTCCTTCTCC GTTGTTGATAGTTTGCGGTT
RAX	Sense Anti-sense	CAACTGGCTACTGTCTGTC CTTATTCCATCTTTCCCACCT
PAX6	Sense Anti-sense	CAGCTCGGTGGTGTCTTTG AGTCGCTACTCTCGGTTTA
RAX-267	Sense	ATTAATAGAGAAGGGGCTGGGT
RAX-525	Sense	ATTAATTAGTCTGAAGTGAGAGG
RAX-1665	Sense Anti-sense	GTCGACGAATTAATCAGAGGTGG AGATCTCTTTGGAGACGGAGAGG
RAX-2761	Anti-sense	AGATCTCTCCCCTTGCGTTTGT
RAX-3007	Anti-sense	AGATCTACCCAGCCCCTTCTC
RAX-3258	Sense Anti-sense	GTCGACGAATTAATCAGAGGTG AGATCTCTTTGGAGACGGAGAGG

ISSN: 2661-8850