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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: SH3BGRL3 binds to myosin 1c in a calcium dependent manner and modulates migration in the MDA-MB-231 cell line

Fig. 3

SH3BGRL3 binds to GAP43 protein in presence of Ca2+. (a) SKBR3 cells were co-transfected with a construct coding for HA-tagged GAP43 and a construct coding for FLAG-SH3BGRL3 or the empty vector. FLAG-SH3BGRL3 and GAP43 were co-immunoprecipitated using an anti-FLAG conjugated resin and GAP43 revealed using an anti-HA antibody. (b) Purified histidine tagged SH3BGRL3 was pulled-down using GST-GAP43 fusion protein or GST alone bound to Sepharose-Glutathione 4B in presence of Ca2+ or EGTA. SH3BGRL3 was revealed using an anti-SH3BGRL3 antibody

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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