Fig. 4

SH3BGRL3 and Myo1c co-localize and interact also in MDA-MB-231 cells. (a) Western blot of MDA-MB-231 and SKBR3 lysates using anti-SH3BGRL3, anti-Myo1c and anti-GAPDH antibodies. (b) Left: two representative confocal microscopy images of FLAG-SH3BGRL3 transfected MDA-MB-231 cells stained with anti-FLAG and anti-Myo1c antibodies. Scale bar = 10 μm. Right: co-localization levels expressed as % SH3BGRL3-positive pixels that were also positive (over threshold) for Myo1c fluorescence, evaluated either on the whole FLAG-SH3BGRL3-transfected cell (left) or on ROIs gating the plasma membrane region (right). (c) FLAG-SH3BGRL3 and Myo1c were co-immunoprecipitated from lysates of FLAG-SH3BGRL3 transfected MDA-MB-231 cells and revealed with anti-FLAG and anti-Myo1c antibodies. Scale bar = 10 μm