Fig. 3

Localisation of the transcript unit of snRNA:7SK:94F gene. a Schema of the region analysed by RT-PCR followed by southern blot. The forward primers (PU14 and PU15) used to perform the reverse transcription step and PCR amplification are located on the schema. The reverse primers (PU10, PU11 and PU12) used to do PCR are also indicated. The probe used to perform hybridization on RT-PCR products is drawn under the gene structure. b Autoradiography of the Southern blot realized with the PU12-PU15 PCR product probe (210nts) onto different RT-PCR products which span the putative transcription unit of snRNA:7SK:94F gene. RT-PU15 indicates that the reverse transcription step has been done with PU15 primers (inside the predicted transcript region) and with PU14 primer (outside the transcript region) for the condition indicated RT-PU14. The last lane is the positive control corresponding to PCR product made with PU12-PU15 primers from the genomic DNA