Fig. 5

snRNA:7SK:94F interacts with P-TEFb. a Co-immunoprecipitation of the protein partners of HEXIM and Cyclin T from Drosophila adult extracts. Western blots using either HEXIM or Larp7 antibodies were performed to analyse the Input, Unbound and Bound fractions. 20% of the input and unbound fractions were loaded onto the gel. b RT-PCR detection of snRNA:7SK:94F (lower panel) and dm7SK (upper panel) RNAs following immunoprecipitation with dmHEXIM and dmCyclin T antibodies from Drosophila adult extracts. RT-PCR reactions were performed with 20% of input and unbound fractions, and with the total bound fraction. UnB: unbound fraction; B: bound fraction; RT: Reverse Transcriptase; Ab: antibody. The size of RT-PCR products or proteins are indicated on the left side of the picture