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Fig. 6 | BMC Molecular and Cell Biology

Fig. 6

From: An alternative D. melanogaster 7SK snRNP

Fig. 6

Direct interaction between snRNA:7SK:94F and dmHEXIM. a Principle of the yeast three-hybrid system, containing three components: the DNA-binding domain of LexA fused to MS2 coat protein, the snRNA:7SK:94F fused to the RNA MS2-binding RNA sequence, and the Gal4 activation domain fused to dmHEXIM protein. When the target hybrid RNA interacts with GAL4-HEXIM protein, the expressions of the reporter genes lacZ and HIS3 were activated. b Qualitative analyses of the interactions between different 7SK RNAs and HEXIMs by monitoring β-galactosidase in situ. For each condition, two representative colonies are shown. h7SK RNA (1–175) is a positive control known to strongly interact with the human HEXIM ortholog (hHEXIM) [41]. c Quantitative analyses of the interactions between dmHEXIM and different sub-domains of snRNA:7SK:94F, either full length (nt 15–404) or shorter fragments (nt 15–163; nt 164–304; and nt 305–404) (lane 1–4). Anti-sense RNAs were used as a negative control (lane 5–8). The β-galactosidase activity was expressed in arbitrary units

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