Fig. 2

MDM2-MDMX binding is essential for MDMX-mediated suppression of p53 protein levels. A MDM2487/487 MEF cells were treated with increasing volumes of MDMX-expressing lentiviral constructs for 48 h prior to lysis and immunoblotting. B, C HEK293 cells were transfected with GFP vector and FLAG-MDM2 along with Myc-tagged MDMX constructs for 24 h prior to lysis and immunoprecipitation (IP). 1 mg of lysate was subject to IP using anti-FLAG beads prior to immunoblotting using an anti-Myc antibody. D MDM2487/487 MEFs were treated with empty vector (EV), lentiviral constructs expressing full-length MDMX, acidic binding domain deletion (ΔAD), or RING domain deletion (ΔRING) MDMX for 48 h prior to lysis and immunoblotting