Fig. 3

The TMD and the cytosolic KKEV motif of Cisd2 cooperate to limit its surface expression. a HEK cells expressing the indicated CD1b-Cisd2 fusion proteins were labeled by immunofluorescence before (Surface) and after (Total) permeabilization, using antibodies specific for the CD1b extracellular domain. All pictures were taken with the same settings with a confocal microscope (LSM700, Zeiss). Scale bar: 10 μm. b The amount of each CD1b fusion protein detected at the cell surface and in the whole cell was quantified. Each dot represents the fluorescence measured in one individual cell. CD1b-M1 was absent from the cell surface, small but significant levels of CD1b-M2 and CD1b-M3 were detected at the cell surface. CD1b-M4 and CD1b-M5 accumulated largely at the cell surface