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Fig. 6 | BMC Molecular and Cell Biology

Fig. 6

From: Intercellular transfer of mitochondrial DNA carrying metastasis-enhancing pathogenic mutations from high- to low-metastatic tumor cells and stromal cells via extracellular vesicles

Fig. 6

Mitochondrial components in A11 S-EVs. (a) Western blot analysis of mitochondrial membrane integrity in A11 S-EVs by using a cocktail of antibodies against mitochondrial proteins. CVa, CIII Core 1, Porin, CypD and Cyt c indicate inner membrane (IM) protein Complex Va, IM protein Complex III Core 1, outer membrane (OM) protein Porin, matrix space protein Cyclophilin D and intermembrane space (IMS) protein Cytochrome c, respectively. Lysates of A11 S-EVs and isolated A11 mitochondria were separated on the same gel. Images of short and long exposure of A11 S-EVs lane were shown. Asterisks show the positions of Porin 1 and 2. Uncropped Western blots images are shown in Fig. S8. (b) Cryo-TEM images of A11 S-EVs. Arrows indicate double membraned structures in S-EVs. Bar: 100 nm. (c) S-EVs isolated from the conditioned medium of MitoTracker Red-stained P29 and A11 cells. Scale bars: 10 μm. (d) Staining of A11 with MitoTracker Red. A11 S-EVs were stained with MitoTracker Red for 1 h in the presence or absence of 10 μM antimycin A. Note that aggregates of S-EVs were observed. Scale bars: 10 μm

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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