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Fig. 7 | BMC Molecular and Cell Biology

Fig. 7

From: Intercellular transfer of mitochondrial DNA carrying metastasis-enhancing pathogenic mutations from high- to low-metastatic tumor cells and stromal cells via extracellular vesicles

Fig. 7

PCR analysis of the presence of mtDNA in S-EVs. (a) Location of the genes in mtDNA used for PCR analysis. (b) Presence of various mtDNA genes (ND1, ND4, ND6, COI, HVR) in A11 S-EVs. (c) PCR-RFLP analysis of the presence of mtDNA with the ND6 G13997A mutation. The ND6 gene 254 bp fragment amplified by PCR using mismatched primers was digested with AflII. The primers were designed to generate 223 bp and 31 bp fragments upon AflII digestion when the G13997A mutation was present. The smaller fragment was not visible. (d) Comparison of the amount of mtDNA in S-EVs isolated from the conditioned medium of P29 cells, A11 cells and MEFs and EVs in B6 serum. Equivalent amounts of EVs (~ 90 ng proteins) were used for PCR amplification of the ND6 gene

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