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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: KDM6A mutations promote acute cytoplasmic DNA release, DNA damage response and mitosis defects

Fig. 4

Cellular back mapping identifies co-localizing NPM1-KDM6A protein populations in nucleoplasm and at extranuclear DNA. A-C Left panel: Scatterplot presentation of KDM6A (green box) and NPM1 (red box) and one intermediate population representing a KDM6A-NPM1 assemblies (orange box). Middle panel: All three populations were mapped back to the cellular compartment. Right panel: Line profile through the nucleus as indicated by an arrow in the image. DAPI (blue), NPM1 (red) and KDM6A variants (green) signals are overlaid. The orange triangles indicate the intermediate populations from the scatterplot. A Analysis of KDM6A WT and NPM1. B Analysis of KDM6A T726K and NPM1. C Analysis of KDM6A JmjC variant with NPM1 in the nucleus and at extranuclear DNA. Scatterplot and back mapping analysis for KDM6A variants and DAPI is shown in Figure S7

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