Fig. 5

Truncated KDM6A variants promote a significant decrease in cells with normal phenotypes in T-24 and SW-1710 cells. A Scoring criteria used to quantifiy the occurrence of nuclear damage. B Frequency of different phenotypes scored as normal interphase (grey) and mitosis (pink), micronuclei (blue) and damage (red) in bi- or mononuclear T-24 and SW-1710 untransfected (UT, 1) cells or transfected with control eGFP (2), eGFP-KDM6A variants WT (3), TPR (4) and JmjC (5) (for simplicity abbreviated as WT, TPR and JmjC). In mononuclear and binuclear cells, the proportion of normal cells decreased significantly for both the eGFP-KDM6A TPR and JmjC compared to eGFP and eGFP-KDM6A WT. Cells with micronuclei were not significantly enriched. This was largely due to an increase in damaged cells. Overall, we observed a non-signifcant trend towards an increased number of binucleated cells. T-test using two-tailed hypothesis, significance levels: * = P ≤ .05, ** = P ≤ 0.01, *** = P ≤ 0.001, see Table S3 for P-values. C Line profiles through lagging chromosomes and the “knot-like structures” of the chromatin bridges found in samples transfected with eGFP-KDM6A TPR (left) or JmjC (right) indicating overlapping signal intensities of KDM6A variants (green) with DNA damage markers RAD51 and p-γH2AX (red)