Fig. 6
From: KDM6A mutations promote acute cytoplasmic DNA release, DNA damage response and mitosis defects
Truncated KDM6A variants decrease the amount of viable in T-24 and SW-1710 cells and exhibit mitosis errors. A FACS analysis in Annexin V based apoptosis assay. Transfected eGFP positive cells were gated based on the threshold obtained from untransfected cells (UT), which were then used to plot PI (for membrane permeability) against Annexin-V-APC (apoptosis marker). The plot was divided into four quadrants, representing the viable population (lower left, grey), early apoptosis (lower right, green), late apoptosis (upper right, dark cyan) and necrosis (upper left, black). B Statistics derived from triplicate measurements. eGFP-KDM6A TPR and JmjC show a significant decrease in cell viability in comparision to the eGFP control in both cell lines. T-test using two-tailed hypothesis, significance levels: * = P ≤ .05, ** = P ≤ 0.01, *** = P ≤ 0.001, see Table S3 for detailed P-values. C Graphic summary of cellular phenotypes observed with KDM6A mutation variants, depicting the impact on localization, mitosis, apoptosis and protein assemblies