Fig. 2

HG treatment induces apoptotic death and activates Fyn and mTOR/S6K in differentiated SH-SY5Y cells. (A) Cleaved caspase 3caspase 3 levels in the HG (75 mM, 6 days) group were higher than those in the control group. The grouping of blots cropped from different parts of the same gel is shown. (B) Cell viability was significantly lower in the HG group than in the control group (n = 5). (C) Annexin V-FITC-positive cells were significantly increased in response to HG treatment compared to controls (n = 3). (D) p-Y416 was obviously higher in the HG group than in the control group (n = 5). The blots of β-actin and Fyn were cropped from different parts of the same gel, and p-Y416 was cropped from different gels. (E) mRNA levels of Fyn were not changed by HG treatment (n = 3) (F) p-mTOR and p-S6K in HG group were significantly higher, but total mTOR and S6K were comparable (n = 5). β-actin and S6K were cropped from different parts of the same gel, and the p-S6K, mTOR and p-mTOR were cropped from different gels