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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Fyn kinase regulates dopaminergic neuronal apoptosis in animal and cell models of high glucose (HG) treatment

Fig. 3

Inhibition of Fyn alleviates HG-induced cell injury by suppressing the mTOR/S6K signaling pathway. (A) Elevated cleaved caspase 3 levels induced by HG were rescued by rapamycin (100 nM) or PP1 (10 μM) treatment. PP1 inhibited p-Y416, but rapamycin did not change p-Y416 levels (n = 5). β-actin, cleaved caspase 3 and Fyn were cropped from different parts of the same gel, and p-Y416 was cropped from a different gel. (B) PP1 or rapamycin inhibited mTOR/S6K (n = 5). β-actin and S6K were cropped from different parts of the same gel, and p-S6K, mTOR and p-mTOR were cropped from different gels. (C) Flow cytometry showed that rapamycin or PP1 decreased the elevated cell apoptotic death induced by HG (n = 3). (D) Decreased cell viability in response to HG was rescued by rapamycin or PP1 administration (n = 5)

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