Phosphate donor | Relative activity |
---|
ATP | 100 |
UTP | 19.1 |
GTP | 23.9 |
CTP | 13.7 |
dATP | 70.7 |
dTTP | 0.10 |
dGTP | 13.9 |
dCTP | 12.4 |
- The assay was performed by using a radiochemical assay with [3H]-dThd as the substrate at 37 °C. The concentration of dThd was 0.1 mM, and the phosphate donor concentration was 1 mM. The data are given as a percentage of that with ATP as a phosphate donor (0.47 μmol/mg/min)