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Table 2 Phosphate donor specificity

From: Molecular characterization of equine thymidine kinase 1 and preliminary evaluation of its suitability as a serum biomarker for equine lymphoma

Phosphate donor

Relative activity

ATP

100

UTP

19.1

GTP

23.9

CTP

13.7

dATP

70.7

dTTP

0.10

dGTP

13.9

dCTP

12.4

  1. The assay was performed by using a radiochemical assay with [3H]-dThd as the substrate at 37 °C. The concentration of dThd was 0.1 mM, and the phosphate donor concentration was 1 mM. The data are given as a percentage of that with ATP as a phosphate donor (0.47 μmol/mg/min)