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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Compression enhances invasive phenotype and matrix degradation of breast cancer cells via Piezo1 activation

Fig. 1

Compression enhanced invasion of MDA-MB-231 cells depending on Piezo1. Cell invasion was measured with in vitro transwell invasion assay. a Schematic diagram of the compression experiment using a transwell setup. Cells grown on a membrane filter (8 μm pore) coated with Matrigel for 6 h were covered with 1% of agarose gel and compressed with a specific weight. b The changes of cell height and nuclear area of MDA-MB-231 under compression. Data are presented as means ± s.e.m. n = 4, ** p < 0.01 versus untreated groups. c, d, e Representative images of invaded cells stained with crystal violet under different compression and treated with gadolinium chloride (Gd3+), GsMTx4, or siRNA for Piezo1 under 400 Pa (Bar = 100 μm). f Quantification of the fold change of invaded cells. Data were presented as means ± s.e.m., n = 3, ** p < 0.01 versus control (Ctr) groups

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