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Table 2 Plasmids used in this study

From: Exportin Crm1 is important for Swi6 nuclear shuttling and MBF transcription activation in Saccharomyces cerevisiae

Plasmid number Genotype Source
pRS315 CEN LEU2 [65]
pKBB353 CEN URA3 SWI61–181-GFP This study
pKBB354 CEN URA3 SWI61–273-GFP This study
pKBB416 CEN URA3 SWI61–273ΔNES-GFP This study
pKBB484 CEN URA3 GAL1::SWI6-GFP This study
pGAL::CCA1-GFP CEN URA3 GAL1::CCA1-GFP [46]
pGAL::H2B-GFP CEN URA3 GAL1::H2B-GFP [46]
pAC1202 CEN TRP1 SWI6-GFP [43]
pAC1571 CEN LEU2 SWI6-GFP [43]
pKBB418 CEN TRP1 SWI6ΔNES-GFP This study
pKBB441 CEN LEU2 SWI6ΔNES-GFP This study
pOBD-2 CEN TRP1 GAL4-BD [56]
pOAD CEN LEU2 GAL4-AD [56]
pKBB486 CEN TRP1 CRM1-BD This study
pKBB491 CEN LEU2 SWI6-AD This study
pBA251 CEN URA3 4X SCB::LacZ [4]
pBA487 CEN URA3 4X MCB::LacZ [4]
pLDB235 CEN URA3 GAL1 L. Davis
pLDB350 CEN URA3 GFP [66]