Fig. 2
From: Nucleolar localization of the ErbB3 receptor as a new target in glioblastoma
Nucleolar localization of ErbB3 is under the control of Neuregulin1. Immunofluorescence analysis for the ErbB3 receptor (green) and B23 (red) in U-87MG cells before and after Neuregulin1 treatment (A). The colocalization is shown in yellow. Scalebar represent 10 μm. Densitometric quantification of the ErbB3 signal in the nucleoli was performed by Image J. U-87MG cell lines were subjected to cell fractionation after 15 min of Neuregulin1 stimulation and analysed by western blot. Whole extract (B), purified nucleoli (C), Nucleoplasm (D) and cytoplasmic extracts (E) were probes with anti ErbB3, anti B23 (only for nucleoli) and specific antibodies for each compartments corresponding to anti Pol I subunit RPA40 for the nucleoli; anti GAPDH for the cytoplasm and anti lamin B1 for the nuclei. The proteins amount loaded in each lane were: whole extract 80 μg, nucleoli, 4 μg; nuclei 10 μg; cytoplasm 40 μg. The quantification data shown in F-H are the average of three independent experiments and significant results are highlighted with asterisks (* p < 0.05, ** p < 0.01)