Fig. 6
From: Nucleolar localization of the ErbB3 receptor as a new target in glioblastoma
Nucleolar localization of ErbB3 in primary cells of glioblastoma. ErbB3 localizes in the nucleolus of cells derived from primary glioblastoma culture (left panel) and re-localizes to the nucleoplasm upon low Actinomycin treatment (right panel) (A). Scalebar represent 50 μm. The specificity of the anti ErbB3 antibody was verified in a primary culture of glioblastoma (GL18–15), in U-87MG, U-251MG, A172 and U-373MG (B). The quantification of the signal is shown in the histogram (B, lower panel). The proteins amount loaded in each lane was 80 μg. The expression of GAFP (C) and ErbB3 (D) were analyzed by qPCR from mRNA purified from patient biopsies. Data are the average of three independent experiments. Significant results are highlighted with asterisks (* p < 0.05)