Fig. 4

Modelling of the EgB4 – inactive EGFR complex. A Structural alignment of EGFR in the inactive, closed (light colors; unmodified from PDB: 3qwq) [41] and active EgB4-bound conformation (dark colors) in ribbon representation. The EgB4 — inactive EGFR complex is modelled by superposition based on the EgB4-EGFR interface from the crystal structure reported here. B Inactive, closed EGFR is also poised to interact with EgB4. Residues involved in the interaction are shown in stick representation. Electrostatic and hydrogen bonding interactions are indicated by black and yellow dotted lines, respectively. Potential steric clashes between EgB4 and residues Arg141 and Ile189 in the inactive EGFR are readily alleviated by selecting different rotamers