Fig. 1

Comparisons between AIRE^WT and AIRE^−/− during the spheroid formation process. A Inverted light microscopy (40 x magnification) showing the spheroid formation in agarose micromolds starting from the deposition of 1 × 10⁵ mTECs, B Spheroid growth curves from 0 to 108 h, time-point values correspond to the mean and ± SD from three independent replicates. C Spheroid cell viability from 0 to 108 h of growth. One representative experiment of n > 3 is shown